中国水稻科学 ›› 2014, Vol. 28 ›› Issue (4): 442-446.DOI: 10.3969/j.issn.1001-7216.2014.04.014

• 实验技术 • 上一篇    

利用四引物扩增受阻突变体系PCR技术检测水稻光温敏核不育基因p/tms12-1

李军* ,李白,高荣村   

  1. 嘉兴市农业科学研究院, 浙江 嘉兴 314016;
  • 收稿日期:2014-01-09 修回日期:2014-02-21 出版日期:2014-07-10 发布日期:2014-07-10
  • 通讯作者: 李军*
  • 基金资助:

    嘉兴市科技计划资助项目(2013BY26006, 2012AZ2021,2013BZ26002)。

Detection of Gene p/tms121  for Photoperiod and ThermoSensitive Genic Male Sterility by TetraPrimer Amplication Refractory Mutation System PCR in Rice

LI  Jun*, LI  Bai, GAO Rongcun   

  1. Jiaxing Academy of Agricultural Sciences, Jiaxing 314016, China; 
  • Received:2014-01-09 Revised:2014-02-21 Online:2014-07-10 Published:2014-07-10
  • Contact: LI Jun*,

摘要: 根据p/tms121基因存在的单核苷酸变异,利用四引物扩增受阻突变体系PCR(Tetraprimer ARMSPCR)的方法设计特异引物,对11个两系不育系水稻品种(或品系)以及2个常规稻品系进行扩增。根据其PCR产物带型,可以准确鉴定光温敏核不育基因p/tms121的基因型,其检测结果与通过CAPS标记检测的结果完全一致。该方法成本低、简单、可靠,可用于p/tms121基因的鉴定和分子标记辅助育种。

关键词: 水稻, 光温敏核不育, p/tms121基因, 四引物扩增受阻突变体系PCR

Abstract: Specific primers were designed for tetraprimer amplification refractory mutation system PCR (ARMSPCR) method according to the single nucleodide mutation in gene p/tms121.  Eleven twoline sterile lines and two normal varieties were analyzed by this method. The results could clearly distinguish the genotype of p/tms121,  which was completely consistent with the result of CAPs markerbased analysis. Therefore, as a lowcost, simple and reliable technique, this method could be widely used to the p/tms121  detection and molecular markerassisted breeding in rice.

Key words: rice, PTGMS, p/tms121, tetraprimer ARMSPCR

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